Merck PSF-TEF1-COOH-EKT-FRCFP-6HIS - C-TERMINAL 6 HIS AND CFP DUAL TAG YEAST PLASMID

PSF-TEF1-COOH-EKT-FRCFP-6HIS

C-terminal 6xHis and CFP Dual Tag Yeast Plasmid

Description

This plasmid is designed for expressing tagged proteins in Saccharomyces cerevisiae.
It includes a URA3 auxotrophic selection cassette for positive selection in uracil-deficient yeast strains, typically grown in minimal media lacking uracil.

Features

• Dual tag: C-terminal 6xHis and FrCFP (cyan fluorescent protein)
• EKT protease cleavage site for tag removal
• Constitutive TEF1 promoter for high expression in yeast
• Kanamycin resistance for bacterial selection
• Suitable for molecular cloning and protein expression studies

Specifications

About the Peptide Tag

This plasmid contains a C-terminal FrCFP fluorescent reporter tag and a secondary 6xHis tag (sequence: HHHHHH).
These dual tags allow both visualization and affinity purification of expressed proteins.
Different peptide tags can provide specific properties such as solubility enhancement, secretion facilitation, or affinity binding.

About the Cleavage Tag

The EKT cleavage site (sequence: DDDDK) allows for removal of tags after purification using Enterokinase.
Cleavage occurs after the lysine residue and will not occur if a proline follows the site (none present in this construct).

Promoter Expression Level

The TEF1 promoter (Yeast Elongation Factor Alpha) is a strong, constitutive yeast promoter requiring no induction.
Alternative promoter options are available (TPI – strong, ADH1 – medium, STE5 – weak, and Galactose-inducible variants) for different expression needs.