Merck PSF-CMV-INTRON1 - INTRON EXPRESSION PLASMID

PSF-CMV-INTRON1 - INTRON EXPRESSION PLASMID

plasmid vector for molecular cloning

expression vector, molecular cloning vector, vector, snapfast vector, plasmid vector, cloning vector, plasmid

form

buffered aqueous solution

분자량

size 4365 bp

박테리아 선정

kanamycin

복제개시점

pUC (500 copies)

펩타이드 절단

no cleavage

프로모터

Promoter name: CMV
Promoter activity: constitutive
Promoter type: mammalian

리포터 유전자

none

배송 상태

ambient

저장 온도

−20°C

PSF-CMV-INTRON1 - INTRON EXPRESSION plasmid contains a chimeric intron in between the promoter (CMV) and the multiple cloning site. Its sequence is a chimera of the first intron of the human beta globin gene and a sequence that was developed by R Reed et al (Genes and Development 1989). It induces efficient splicing in eukaryotic cells. We have found that when using a strong promoter in in vitro assays the intron does not increase expression however it has been reported that the presence of an intron can increase the expression level of weaker promoters and can also increase the duration of expression in vivo.

Promoter Expression Level: PSF-CMV-INTRON1 - INTRON EXPRESSION plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture.