Merck PSF-CMV-NH2-INSULINSP-NCOI - MAMMALIAN SECRETION PLASMID

PSF-CMV-NH2-INSULINSP-NCOI - MAMMALIAN SECRETION PLASMID

plasmid vector for molecular cloning

expression vector, molecular cloning vector, vector, snapfast vector, plasmid vector, cloning vector, plasmid

form

buffered aqueous solution

분자량

size 4292 bp

박테리아 선정

kanamycin

복제개시점

pUC (500 copies)

펩타이드 절단

no cleavage

펩타이드 태그 위치

N-terminal

프로모터

Promoter name: CMV
Promoter activity: constitutive
Promoter type: mammalian

리포터 유전자

none

배출 신호

human Insulin

배송 상태

ambient

저장 온도

−20°C

This plasmid adds the secretory signal peptide that normally induces the secretion of insulin in humans. It will traffic any protein to which it is attached at the N-terminus into the endoplasmic reticulum. It is positioned upstream of the multiple cloning site but adjacent to the NcoI restriction site. If your protein has no organelle retention signals or specific trafficking sequences it will most likely be secreted into the medium by bulk flow exocytosis mechanisms.

This tag will translocate the protein to which it is attached into the endoplasmic reticulum (ER) of a eukaryotic cell after which the signal peptide will be cleaved off. The signals that traffic the protein to the ER are within the tag as are the signals that mediate its cleavage from the downstream protein. Its activity is based more on the hydropathy of the signal peptide sequence rather than the actual order of the amino acids within it. The amino acid sequence of the signal peptide is MALWMRLLPLLALLALWGPDPAAA. Cleavage occurs immediately after the final alanine residue.

Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture.