Merck PSF-CMV-NH2-HA-EKT-NCOI - N-TERMINAL FLU HA TAG PLASMID

PSF-CMV-NH2-HA-EKT-NCOI - N-TERMINAL FLU HA TAG PLASMID

plasmid vector for molecular cloning

expression vector, molecular cloning vector, vector, snapfast vector, plasmid vector, cloning vector, plasmid

태그

Hemagglutinin A (HA) tagged

form

buffered aqueous solution

분자량

size 4265 bp

박테리아 선정

kanamycin

복제개시점

pUC (500 copies)

펩타이드 절단

no cleavage

펩타이드 태그 위치

N-terminal

프로모터

Promoter name: CMV
Promoter activity: constitutive
Promoter type: mammalian

리포터 유전자

none

배송 상태

ambient

저장 온도

−20°C

This plasmid adds a HA epitope tag to the N-terminus of a protein that is encoded within the multiple cloning site. This tag allows the detection and purification of a tagged protein using antibodies raised against the Influenza HA epitope. The HA tag coding sequence is YPYDVPDYA. There is an enterokinase cleavage site (DDDDK) immediately downstream of the HA tag that can be used to remove the HA tag from a purified protein. It cleaves after the lysine residue.

Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture.