Merck PSF-EF1-UB-NEO/G418 ASCI - EF1 ALPHA PROMOTER G418 SELECTION PLASMID

PSF-EF1-UB-NEO/G418 ASCI - EF1 ALPHA PROMOTER G418 SELECTION PLASMID

plasmid vector for molecular cloning

expression vector, molecular cloning vector, vector, snapfast vector, plasmid vector, cloning vector, plasmid

form

buffered aqueous solution

분자량

size 6969 bp

박테리아 선정

kanamycin

포유류 세포 선정

puromycin

복제개시점

pUC (500 copies)

펩타이드 절단

no cleavage

프로모터

Promoter name: EF1-alpha
Promoter activity: constitutive
Promoter type: mammalian

리포터 유전자

none

배송 상태

ambient

저장 온도

−20°C

This plasmid vector contains the EF1alpha promoter upstream of the MCS and Neomycin/G418 resistance driven by the ubiquitin promoter

Promoter Expression Level: This plasmid contains the human Elongation factor 1 promoter to drive protein expression. The EF1 alpha promoter contains a large intron that helps to maintain expression of the promoter in long term culture. Although it shows signficantly lower expression in most cell types compared to the CMV promoter by transient transfection the promoter can maintain activity for longer in many cell types when making stable cell lines. The ubiquitin promoter driving the G418 resistance gene demonstrates similiar levels of expression to the EF1 alpha promoter in most cell types. The Ubiquitin promoter contains a large intron that helps to maintain promoter activity.