Merck PSF-CMV-UB-PURO ASCI-EMCV-FLUC - EMCV IRES LUCIFERASE PUROMYCIN SELECTION VECTOR

PSF-CMV-UB-PURO ASCI-EMCV-FLUC - EMCV IRES LUCIFERASE PUROMYCIN SELECTION VECTOR

plasmid vector for molecular cloning

expression vector, molecular cloning vector, vector, snapfast vector, plasmid vector, cloning vector, plasmid

form

buffered aqueous solution

분자량

size 8368 bp

박테리아 선정

kanamycin

포유류 세포 선정

puromycin

복제개시점

pUC (500 copies)

펩타이드 절단

no cleavage

프로모터

Promoter name: CMV
Promoter activity: constitutive
Promoter type: mammalian

리포터 유전자

firefly luciferase

배송 상태

ambient

저장 온도

−20°C

This vector contains the CMV promoter upstream of the internal ribosoms entry site (IRES) from Encephalomyocarditis virus (EMCV) which is driving the expression of the firefly luciferase reporter gene. The vector also contains a puromycin selection cassette that allows the production of stable cell lines in mammalian cells. Transgenes can be inserted upstream of teh EMCV IRES sequence using our standard multiple cloning site. This will allow both the gene of interest and the luciferase to be produced from the same mRNA molecule.

Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture. The human ubiquitin promoter driving reporter gene expression is the strongest mammalian endogenous promoters that we provide for many cell types. The IRES that is used to drive luciferase expression from the CMV promoter is considerably weaker than the CMV promoter and typically yields 15-20 fold lower protein levels.