Merck PSF-PGK-EMCV-PURO - PGK PROMOTER EMCV IRES PUROMYCIN VECTOR

PSF-PGK-EMCV-PURO - PGK PROMOTER EMCV IRES PUROMYCIN VECTOR

plasmid vector for molecular cloning

expression vector, molecular cloning vector, vector, snapfast vector, plasmid vector, cloning vector, plasmid

form

buffered aqueous solution

분자량

size 4172 bp

박테리아 선정

kanamycin

포유류 세포 선정

puromycin

복제개시점

pUC (500 copies)

펩타이드 절단

no cleavage

프로모터

Promoter name: PGK
Promoter activity: constitutive
Promoter type: mammalian

리포터 유전자

none

배송 상태

ambient

저장 온도

−20°C

This plasmid vector contains the phosphoglycerate kinase (PGK) promoter upstream of the MCS and EMCV IRES regulating expression of puromycin resistance

Promoter Expression Level: The murine phosphoglycerate kinase (PGK) promoter driving expression allows for long term stable expression in vitro and in vivo in most types. The IRES that is used to drive puromycin resistance downstream of the PGK promoter produces significantly weaker expression compared to the upstream gene (typically 15-20 fold lower than the upstream gene).