Merck PSF-OXB20-SNAPFUSION - BACTERIAL SNAPFUSION CONSTIUTIVE PLASMID

PSF-OXB20-SNAPFUSION - BACTERIAL SNAPFUSION CONSTIUTIVE PLASMID

plasmid vector for molecular cloning

expression vector, molecular cloning vector, vector, snapfast vector, plasmid vector, cloning vector, plasmid

재조합

expressed in E. coli

form

buffered aqueous solution

분자량

size 3866 bp

박테리아 선정

kanamycin

복제개시점

pUC (500 copies)

펩타이드 절단

no cleavage

프로모터

Promoter name: OXB20
Promoter activity: constitutive
Promoter type: bacterial

리포터 유전자

none

배송 상태

ambient

저장 온도

−20°C

This vector is a standard E. coli expression plasmid with the exception that it has been modified to allow the direct cloning of gene fragments that have been designed to fit with any of our peptide tag plasmid range. This can be useful if you have created a peptide fusion but you would also like to create a non-tagged gene variant. The multiple cloning site contains two BsgI and BseRI sites that produce overhangs that are compatible with gene designed using our SnapFusion primer designer (see the cloning tab for this product). The promoter in this plasmid is a strong constitutive bacterial promoter that does not require induction for activity.

Promoter Expression Level: This plasmid contains a very strong constitutive E. coli promoter that was derived from the RecA promoter by removing the LexA repressor site. It is part of our constitutive bacterial promoter range. This promoter shows the highest level of expression in the promoter range with OXB1 showing the lowest level. They require no inducing agent for expression.