Merck PSF-T7-T7 TERM - T7 SINGLE TERMINATOR PLASMID

PSF-T7-T7 TERM - T7 SINGLE TERMINATOR PLASMID

plasmid vector for molecular cloning

expression vector, molecular cloning vector, vector, snapfast vector, plasmid vector, cloning vector, plasmid

form

buffered aqueous solution

분자량

size 3448 bp

박테리아 선정

kanamycin

복제개시점

pUC (500 copies)

펩타이드 절단

no cleavage

프로모터

Promoter name: T7
Promoter type: phage

리포터 유전자

none

배송 상태

ambient

저장 온도

−20°C

PSF-T7-T7 TERM - T7 SINGLE TERMINATOR PLASMID allows transcription of RNA using the T7 bacteriophage polymerase. If you using an in vitro transcription kit the RNA produced from this vector will be uncapped and poly-adenylated making it unsuitable for mammalian cells however some kits are available that will add these features. This plasmid can also be used for inducible gene expression in bacterial cell lines that express the T7 polymerase.

This expression vector contains the T7 terminator only whereas most of our vectors contain mammalian T7 and bacterial termination signals together. This plasmid is normally only used if the presence of the mammalian and bacterial terminators is undesirable.

This molecular cloning vector can also be digested at a restriction site that is 3 prime to the end of your gene to allow run-off of the polymerase rather than exploiting the hairpin T7 terminator. If you are expressing your gene in a Vaccinia virus-free mammalian T7 system it will require an IRES upstream of your gene because of the absence of a 5 prime cap on the RNA produced please see OG85: pSF-T7-EMCV if this is required.