Merck PSF-T7/LACO - T7 LACO INDUCIBLE BACTERIAL PLASMID

PSF-T7/LACO - T7 LACO INDUCIBLE BACTERIAL PLASMID

plasmid vector for molecular cloning

expression vector, molecular cloning vector, vector, snapfast vector, plasmid vector, cloning vector, plasmid

form

buffered aqueous solution

분자량

size 3883 bp

박테리아 선정

kanamycin

복제개시점

pUC (500 copies)

펩타이드 절단

no cleavage

프로모터

Promoter name: T7 Lac
Promoter activity: inducible
Promoter type: phage

리포터 유전자

none

배송 상태

ambient

저장 온도

−20°C

PSF-T7/LACO - T7 LACO inducible bacterial plasmid contains T7 promoter that can be regulated using the Lac repressor. The T7 LACO plasmid allows greater control over expression in comparison to normal T7 promoter plasmids. In order to use this inducible bacterial plasmid you will require a T7 polymerase expressing E. coli cell line that also expresses the LacI repressor. A common expression cell line that can be used for this purpose is the BL21 (DE3) strain.

Promoter Expression Level: This plasmid contains the T7 promoter followed by the lac operon operator sequence. This promoter is useful for inducible expression in bacterial cells that express the T7 polymerase.