Merck PSF-CMV-UB-ILUMENA - MAMMALIAN SECRETED LUCIFERASE VECTOR

PSF-CMV-UB-ILUMENA - MAMMALIAN SECRETED LUCIFERASE VECTOR

plasmid vector for molecular cloning

expression vector, molecular cloning vector, vector, snapfast vector, plasmid vector, cloning vector, plasmid

form

buffered aqueous solution

분자량

size 6183 bp

박테리아 선정

kanamycin

복제개시점

pUC (500 copies)

펩타이드 절단

no cleavage

프로모터

Promoter name: CMV
Promoter activity: constitutive
Promoter type: mammalian

리포터 유전자

iLumena luciferase

배송 상태

ambient

저장 온도

−20°C

This plasmid contains the CMV promoter upstream of our standard multiple cloning site to allow the production of proteins in mammalian cells. The vector also contains a secreted luciferase reporter gene that is away from the multiple cloning site. The reporter is unique to our plasmids and exhibits high levels of luminescence in the supernantent of eukaryotic cells. The substrate for the luciferase is coelenterazine.

Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture. The human ubiquitin promoter driving reporter gene expression is the strongest mammalian endogenous promoters that we provide for many cell types.