Merck PSF-CAG-BETAGAL-UB-PURO - CAG REPORTER (BETA GAL) PLASMID

PSF-CAG-BETAGAL-UB-PURO - CAG REPORTER (BETA GAL) PLASMID

plasmid vector for molecular cloning

expression vector, molecular cloning vector, vector, snapfast vector, plasmid vector, cloning vector, plasmid

form

buffered aqueous solution

분자량

size 10450 bp

박테리아 선정

kanamycin

포유류 세포 선정

puromycin

복제개시점

pUC (500 copies)

펩타이드 절단

no cleavage

프로모터

Promoter name: CAG
Promoter activity: constitutive
Promoter type: mammalian

리포터 유전자

beta Gal

배송 상태

ambient

저장 온도

−20°C

PSF-CAG-BETAGAL-UB-PURO - CAG REPORTER (BETA GAL) PLASMID s designed for creating beta galactosidase stable cell lines or the transient expression on beta galactosidase. It contains the strong constitutive mammalian CAG promoter to drive the expression of the beta galactosidase reporter and a puromycin resistance gene to allow for stable cell line selection.

Promoter Expression Level: This expression vector contains the mammalian CAG promoter which is a synthetic composite of the CMV immediate early enhancer followed by the CBA promoter and the rabbit beta globin intron. The Chicken beta actin contains a CpG island that can help to keep the promoter active for longer in stable culture when compared to the CMV promoter. The ubiquitin promoter that is driving expression of the puromycin gene demonstrates a high level of expression in most cell types. This cloning vector demonstrates similiar levels of expression to the commonly used EF1-Alpha promoter.