Merck PSF-CMV-UB-BLAST ASCI - UBIQUITIN PROMOTER BLASTICIDIN PLASMID

PSF-CMV-UB-BLAST ASCI - UBIQUITIN PROMOTER BLASTICIDIN PLASMID

plasmid vector for molecular cloning

expression vector, molecular cloning vector, vector, snapfast vector, plasmid vector, cloning vector, plasmid

form

buffered aqueous solution

분자량

size 5981 bp

박테리아 선정

kanamycin

포유류 세포 선정

blasticidin

복제개시점

pUC (500 copies)

펩타이드 절단

no cleavage

프로모터

Promoter name: CMV
Promoter activity: constitutive
Promoter type: mammalian

리포터 유전자

none

배송 상태

ambient

저장 온도

−20°C

This vector contains a blasticidin (Blast) resistance expression cassette under control of the Ubiquitin (Ub) promoter that is designed as an add-on to any of our vectors. The entire Ub Blast cassette is flanked by AscI sites. The Ub promoter is approximately 10-fold weaker than CMV. We also have have a weaker Rous Sarcoma Virus LTR promoter driven vector (pSF-CMV-RSV-Blast AscI) in the same format if you require even lower levels of blasticidin resistance.

Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture.