Merck PSF-OXB20-COOH-EKT-FLAG - C-TERMINAL FLAG TAG BACTERIAL PLASMID

PSF-OXB20-COOH-EKT-FLAG® - C-TERMINAL FLAG® TAG BACTERIAL PLASMID

plasmid vector for molecular cloning

expression vector, molecular cloning vector, vector, snapfast vector, plasmid vector, cloning vector, plasmid

재조합

expressed in E. coli

태그

FLAG® tagged

form

buffered aqueous solution

분자량

size 3893 bp

박테리아 선정

kanamycin

복제개시점

pUC (500 copies)

펩타이드 절단

EKT

펩타이드 태그 위치

C-terminal

프로모터

Promoter name: OXB20
Promoter activity: constitutive
Promoter type: bacterial

리포터 유전자

none

배송 상태

ambient

저장 온도

−20°C

This plasmid is designed to express tagged proteins in E. coli. The plasmid contains a constitutive promoter (OXB20) derived from the region upstream of the E. coli RecA gene. It does not require induction or any additional components for activity. It is the strongest of the bacterial promoters that we provide and this high level of expression can cause expression problems with some proteins with poor solubility. For this reason we sell a range of bacterial promoters with different expression levels (OXB1(low)>OXB20(high)) that can be provided with the peptide tags in this plasmid on request.

About the Peptide Tag:This plasmid contains a c-terminal Flag epitope tag that can be fused to a gene of interest to allow protein detection and/or purification. The sequence of the tag is: DYKDDDDK.

About the Cleavage Tag:This plasmid also encodes a protease cleavage site that is designed to be positioned between your gene of interest and the tag to allow the removal of the tag following protein purification or isolation. This plasmid contains a EKT cleavage tag. The protein sequence of the cleavage tag is: DDDDK. Enterokinase (EKT) protease cleaves after the Lysine residue. It can cleave at other basic residues but this is dependent on protein confirmation. If a proline follows the site it will not cut. None of our products contain a proline after the site.

Promoter Expression Level: This plasmid contains a constitutive bacterial promoter that does not require induction. It is the strongest bacterial promoter we sell and this can cause solubility and expression problems with some proteins. We also offer a range of other bacterial promoters that are compatible with this plasmid and are available on request.