Merck pSF-CMV-NEO-NH2-PPT-3XFLAG-COOH-Cmyc

pSF-CMV-NEO-NH2-PPT-3XFLAG-COOH-Cmyc

plasmid vector for molecular cloning

expression vector, molecular cloning vector, vector, snapfast vector, plasmid vector, cloning vector, plasmid

재조합

expressed in human cells

태그

3X FLAG tagged
c-Myc tagged

form

buffered aqueous solution

분자량

size 5973 bp

박테리아 선정

ampicillin

복제개시점

pUC

펩타이드 절단

EKT

펩타이드 태그 위치

C-terminal
N-terminal

프로모터

Promoter name: CMV
Promoter activity: constitutive
Promoter type: mammalian

배송 상태

ambient

저장 온도

−20°C

The pSF-CMV-NEO-NH2-PPT-3XFLAG-COOH-Cmyc expression vector is a 6 kb derivative of pSF-CMV-Amp for transient or stable expression of secreted dual-tagged N-terminal 3XFLAG and C-terminal c-myc fusion proteins in mammalian cells. This vector encodes three FLAG epitopes in tandem (DYKDHDG-DYKDHDI-DYKDDDDK), which results in increased sensitivity when using the anti-FLAG M2 antibody (catalogue number F3165). The third epitope includes the enterokinase recognition sequence, allowing cleavage of the 3XFLAG peptide from the purified fusion protein. The preprotrypsin (PPT) leader sequence precedes the 3XFLAG sequence and directs secretion of the fusion protein into the culture medium. A c-myc epitope tag (EQKLISEEDL) is present downstream of the multiple cloning site. pSF-CMV-NEO-NH2-PPT-3XFLAG-COOH-Cmyc is a shuttle vector, containing both E. coli and SV40 origins of replication, for propagation in bacterial and mammalian cells. Efficiency of replication and genomic integration is optimal when using an SV40 T antigen expressing mammalian host. The promoter regulatory region of the human cytomegalovirus drives transcription of FLAG and c-myc fusion constructs. The aminoglycoside phosphotransferase II gene (neo-R) confers resistance to aminoglycosides such as G418, allowing for selection of stable transfectants.