Merck pSF-T7LacO-NH2-FLAG

pSF-T7LacO-NH2-FLAG

plasmid vector for molecular cloning

expression vector, molecular cloning vector, vector, snapfast vector, plasmid vector, cloning vector, plasmid

재조합

expressed in E. coli

태그

FLAG® tagged

form

buffered aqueous solution

분자량

size 5496 bp

박테리아 선정

ampicillin

복제개시점

pUC

펩타이드 절단

EKT

펩타이드 태그 위치

N-terminal

프로모터

Promoter name: T7 Lac
Promoter activity: inducible
Promoter type: bacterial

배송 상태

ambient

저장 온도

−20°C

The pSF-T7LacO-NH2-FLAG expression vector is a 5.5 kb E. coli expression vector used for cloning and cytoplasmic expression of a properly inserted open reading frame as an N-terminal Met-FLAG fusion protein. The FLAG epitope is a small- hydrophilic- 8 amino acid-tag (DYKDDDDK) that enables sensitive detection and high quality protein purification using anti-FLAG products. The N-terminal Met-FLAG fusion protein may be detected using monoclonal anti-FLAG M1 (catalogue number F3040) or M2 (F3165) antibodies. The fusion protein may be purified using anti-FLAG affinity gel (A2220). In this vector, transcription of the fusion protein is driven by the strong phage T7 promoter. This requires the use of E. coli cells containing a source of the T7 polymerase, such as BL21(DE3)T1R cells (catalogue number B2935).