Merck OverExpress C43(DE3) pLysS Chemically Competent Cells

OverExpress™ C43(DE3) pLysS Chemically Competent Cells

for the highest protein expression

Grade

for molecular biology

세포 형질전환

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제품 설명

OverExpress™ Electrocompetent and Chemically Competent Cells are E. coli strains effective in expressing toxic proteins from various organisms, including eubacteria, yeasts, plants, viruses, and mammals.
Their effectiveness has been validated in more than 350 publications.
These strains contain genetic mutations selected to confer tolerance to toxic proteins.

The strain C41(DE3) was derived from BL21(DE3) and carries a mutation preventing cell death associated with recombinant toxic protein expression.
C43(DE3) was derived from C41(DE3) by selecting for resistance to a different toxic protein and can express a different set of toxic proteins.

OverExpress™ C41(DE3)pLysS and C43(DE3)pLysS also carry a chloramphenicol-resistant plasmid encoding T7 lysozyme, a natural inhibitor of T7 RNA polymerase.
Cells containing pLysS produce a small amount of T7 lysozyme, suppressing basal expression of T7 RNA polymerase prior to induction and stabilizing recombinants encoding particularly toxic proteins.

Genotype