Merck PSF-MINCMV-SEAP - ALKALINE PHOSPHATASE MINIMAL CMV PLASMID

PSF-MINCMV-SEAP - ALKALINE PHOSPHATASE MINIMAL CMV PLASMID

plasmid vector for molecular cloning

expression vector, molecular cloning vector, vector, snapfast vector, plasmid vector, cloning vector, plasmid

form

buffered aqueous solution

분자량

size 5296 bp

박테리아 선정

kanamycin

복제개시점

pUC (500 copies)

펩타이드 절단

no cleavage

프로모터

Promoter name: CMV
Promoter activity: minimal promoter
Promoter type: mammalian

리포터 유전자

SEAP

배송 상태

ambient

저장 온도

−20°C

This plasmid has the minimal CMV promoter regulating expression of secreted alkaline phosphatase (SEAP) allowing low levels of expression in most mammalian cells. The activity of the minimal promoter can be tuned using a specially-positioned upstream MCS to introduce sites that respond to externally-applied drugs or cell selective (stimulatory or repressive) transcription factors providing a diverse range of possible reporter systems. Transgene expression can be measured without cell lysis as the SEAP is secreted from the transfected cell.

Promoter Expression Level: This plasmid contains the minimal CMV immediate early promoter with a multiple cloning site immediately upstream that allows transcription factor binding sites to be inserted. This allows tissue specific or physiologically responsive promoters to be created.