Merck PSF-CMV-NEO/G418 - G418 SELECTION CMV EXPRESSION PLASMID

PSF-CMV-NEO/G418 - G418 SELECTION CMV EXPRESSION PLASMID

plasmid vector for molecular cloning

expression vector, molecular cloning vector, vector, snapfast vector, plasmid vector, cloning vector, plasmid

form

buffered aqueous solution

분자량

size 5005 bp

박테리아 선정

kanamycin

포유류 세포 선정

neomycin / G418

복제개시점

pUC (500 copies)

펩타이드 절단

no cleavage

프로모터

Promoter name: CMV
Promoter activity: constitutive
Promoter type: mammalian

리포터 유전자

none

배송 상태

ambient

저장 온도

−20°C

In this resistance construct the Neomycin/G418 resistance gene is regulated by the CMV promoter allowing simple drug-based selection of transfected cells.

Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture.