Merck PSF-OXB20-BETAGAL - BACTERIAL BETA GALACTOSIDASE PLASMID

PSF-OXB20-BETAGAL - BACTERIAL BETA GALACTOSIDASE PLASMID

plasmid vector for molecular cloning

expression vector, molecular cloning vector, vector, snapfast vector, plasmid vector, cloning vector, plasmid

form

buffered aqueous solution

분자량

size 7371 bp

박테리아 선정

kanamycin

복제개시점

pUC (500 copies)

펩타이드 절단

no cleavage

프로모터

Promoter name: OXB20
Promoter activity: constitutive
Promoter type: bacterial

리포터 유전자

beta Gal

배송 상태

ambient

저장 온도

−20°C

The expression of the beta galactosidase (beta gal) reporter gene under the control of the RecA constitutive bacterial promoter. This plasmid can be used for monitoring reporter gene activity in bacterial cells or colonies using X-Gal which will produce a blue colouration. The reporter gene has been inserted thin the primary multiple cloning site. X-Gal is not membrane permeable and so cells must be lysed prior to detect when screening for blue colonies on an agar plate sufficient cells will naturally lyse on the plate to allow blue colony detection.

Promoter Expression Level: This plasmid contains a constitutive bacterial promoter that does not require induction. It is the strongest bacterial promoter we sell and this can cause solubility and expression problems with some proteins. We also offer a range of other bacterial promoters that are compatible with this plasmid and are available on request.