Merck PSF-CAG-UB-PURO - CAG PROMOTER PUROMYCIN RESISTANT VECTOR

PSF-CAG-UB-PURO - CAG PROMOTER PUROMYCIN RESISTANT VECTOR

plasmid vector for molecular cloning

expression vector, molecular cloning vector, vector, snapfast vector, plasmid vector, cloning vector, plasmid

form

buffered aqueous solution

분자량

size 7265 bp

박테리아 선정

kanamycin

포유류 세포 선정

puromycin

복제개시점

pUC (500 copies)

펩타이드 절단

no cleavage

프로모터

Promoter name: CAG
Promoter activity: constitutive
Promoter type: mammalian

리포터 유전자

none

배송 상태

ambient

저장 온도

−20°C

In this vector the CAG promoter is upstream of the MCS (where your gene of interest can be easily inserted) and puromycin resistance gene is under control of the ubiquitin promoter. CAG is a synthetic composite of the CMV immediate early enhancer followed by the CBA promoter and the rabbit beta globin intron and provide high level and durable expression in mammalian cells.

Promoter Expression Level: This plasmid contains the mammalian CAG promoter which is a synthetic composite of the CMV immediate early enhancer followed by the CBA promoter and the rabbit beta globin intron. The Chicken beta actin contains a CpG island that can help to keep the promoter active for longer in stable culture when compared to the CMV promoter. The ubiquitin promoter that is driving expression of the puromycin gene demonstrates a high level of expression in most cell types. It demonstrates similiar levels of expression to the commonly used EF1-Alpha promoter.