Merck PSF-CMV-PURO-NH2-INSULINSP - INSULIN SECRETION PLASMID

PSF-CMV-PURO-NH2-INSULINSP - INSULIN SECRETION PLASMID

plasmid vector for molecular cloning

expression vector, molecular cloning vector, vector, snapfast vector, plasmid vector, cloning vector, plasmid

form

buffered aqueous solution

분자량

size 6215 bp

박테리아 선정

kanamycin

포유류 세포 선정

puromycin

복제개시점

pUC (500 copies)

펩타이드 절단

no cleavage

펩타이드 태그 위치

N-terminal

프로모터

Promoter name: CMV
Promoter activity: constitutive
Promoter type: mammalian

리포터 유전자

none

배출 신호

human Insulin

배송 상태

ambient

저장 온도

−20°C

This plasmid is designed to express tagged proteins in mammalian cells either by transient transfection or by creating stable cell lines. It contains a puromycin resistance expression cassette using the human Ubiquitin promoter to drive expression and allow for the selection of cells containing the plasmid.

About the Peptide Tag:This plasmid contains an Human Insulin (iSP) secretory signal peptide (SP) to allow proteins to be exported from the cytosol. During translocation from the cytosol the signal peptide is removed from the protein by endogenous proteases.

Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture.