Merck Hanahan′s Broth (SOB Medium)

Hanahan′s Broth (SOB Medium)

powder microbial growth medium

Quality Level

100

Grade

for molecular biology

설명

recommended for cultivation of recombinant E. coli.

무균

non-sterile

form

powder

구성

KCl, 0.186 g/L
MgSO4, 2.4 g/L
NaCl, 0.5 g/L
Tryptone, 20 g/L
Yeast extract, 5 g/L

pH

6.8-7.2(2.8% solution)

application(s)

food and beverages

저장 온도

room temp

적합성

nonselective for Escherichia coli
nonselective for coliforms

Hannahan′s Broth (SOB) is a microbial growth medium used for the preparation and transformation of competent cells (E. coli). This nutrient-rich microbial broth contains peptides, amino acids, and water-soluble vitamins in a low-salt formulation. The 0.4% dextrose composition provides the E. coli carbon and energy needed to mend perforations and for replication.