Merck Auramine O

Auramine O

Dye content ≥80 %, certified by the Biological Stain Commission

Pyoctaninum aureum, 4,4′-(Imidocarbonyl)bis(_N,N_-dimethylaniline) monohydrochloride, Basic Yellow 2

Quality Level

200

Grade

certified by the Biological Stain Commission

form

powder

구성

Dye content, ≥80%

mp

>250 °C (dec.) (lit.)

solubility

ethanol: 1 mg/mL, yellow to orange

λmax

370 nm in H2O
432 nm in H2O

application(s)

diagnostic assay manufacturing
hematology
histology

저장 온도

room temp

SMILES string

Cl[H].CN(C)c1ccc(cc1)C(=N)c2ccc(cc2)N(C)C

InChI

1S/C17H21N3.ClH/c1-19(2)15-9-5-13(6-10-15)17(18)14-7-11-16(12-8-14)20(3)4;/h5-12,18H,1-4H3;1H

InChI key

KSCQDDRPFHTIRL-UHFFFAOYSA-N

Certified for use by fluorescence microscopy in Churukian′s modification of Truant′s fluorescent method for acid fast bacilli on paraffin sections.

Auramine O is used for the staining of acid-fast organisms like coccidia. Along with carbol, it generates brilliant yellow fluorochrome of tubercle bacilli. It is effective in detecting positive cases of tuberculosis. Auramine O binds to the mycolic acid in the bacterial cell wall.