Merck Fluorescein isothiocyanate isomer I

Fluorescein isothiocyanate isomer I

suitable for protein labeling, ≥90% (HPLC), powder

FITC, Fluorescein 5-isothiocyanate

Quality Level

200

assay

≥90% (HPLC)

form

powder

mp

>360 °C (lit.)

solubility

acetone: 1 mg/mL

형광

λex 492 nm; λem 518 nm (green)

적합성

suitable for protein labeling

application(s)

diagnostic assay manufacturing
hematology
histology

저장 온도

2-8°C

SMILES string

Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(ccc45)N=C=S)c1

InChI

1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H

InChI key

MHMNJMPURVTYEJ-UHFFFAOYSA-N

Fluorescein isothiocyanate (FITC) is yellow-orange in color with an absorption maximum at 495nm. Upon excitation, it emits a yellow-green color with an emission maximum at 525nm.
It is widely used to attach a fluorescent label to proteins via the amine group. The isothiocyanate group reacts with amino terminal and primary amines in proteins. It has been used for the labeling of proteins including antibodies and lectins.
Fluorescein isothiocyanate isomer I has been proposed as a contact sensitizer.