Merck Platelet-Derived Endothelial Cell Growth Factor human

Platelet-Derived Endothelial Cell Growth Factor human

recombinant, expressed in baculovirus infected _Sf_21 cells, lyophilized powder, suitable for cell culture

PD-ECGF

Quality Level

200

생물학적 소스

human

재조합

expressed in baculovirus infected _Sf_21 cells

assay

≥97% (SDS-PAGE)

form

lyophilized powder

효능

5-30 ng/mL EC50

분자량

49 kDa

포장

pkg of 10 μg

저장 조건

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

불순물

<1 EU/mg

UniProt 수납 번호

P01127

저장 온도

−20°C

Gene Information

human ... PDGFA(100126841) , TYMP(1890)

Platelet-Derived Endothelial Cell Growth Factor (PD-ECGF) is an endothelial cell mitogen which was originally purified from human platelets. In contrast with the endothelial mitogens of the fibroblast growth factor (FGF) family, PD-ECGF is an endothelial cell growth factor initially purified from human platelets, which does not bind to heparin and does not stimulate the proliferation of fibroblasts. This is in contrast with the effect of PDGF, which stimulates the growth of human foreskin fibroblasts, but is inactive on endothelial cells. PD-ECGF will stimulate endothelial cells in vitro and in vivo. PD-ECGF is chemotactic for bovine aortic endothelial cells. PD-ECGF does not induce smooth muscle cell migration. PD-ECGF is involved in angiogenesis and has potent angiogenic activity both in the developing vascular system of the chick chorioallantoic membrane and in vascularization of tumors in nude mice. PD-ECGF has a pI of 4.0-4.8 and a molecular weight of 49 kDa.

The biological activity of recombinant, human PD-ECGF is measured in a cell proliferation assay using human umbilical vein endothelial cells. The EC50 is defined as the effective concentration of growth factor that elicits a 50% increase in cell growth in a cell based bioassay