Merck Granulocyte-Macrophage Colony-Stimulating Factor from mouse

Granulocyte-Macrophage Colony-Stimulating Factor from mouse

GM-CSF, from mouse, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

GM-CSF

Quality Level

200

생물학적 소스

mouse

재조합

expressed in E. coli

assay

≥97% (SDS-PAGE)

form

lyophilized powder

효능

≤0.2 ng/mL EC50 (corresponds to ≥5 × 106 units/mg)

품질

endotoxin tested

분자량

~15 kDa (125 amino acids including N-terminal methionine)

포장

pkg of 5 and 50 μg

저장 조건

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

불순물

≤1 EU/mg

색상

white

solubility

water: soluble, clear, colorless

UniProt 수납 번호

P01587

저장 온도

−20°C

Gene Information

mouse ... CSF2(12981) , Csf2(12981)

Granulocyte-macrophage colony stimulating factor (GM-CSF) is a growth and differentiation factor for cells in the granulocyte, macrophage and eosinophil lineage. GM-CSF stimulates colony formation from pluripotential progenitor cells at extremely low concentrations and is an essential survival and proliferative factor for hematopoietic progenitor cells in all divisions up to maturity. It also stimulates growth in some epithelial cells and osteoclasts. GM-CSF is produced by a variety of cell types (monocytes, endothelial cells, T-cells, fibroblasts, mitogen-stimulated B-cells, and LPS-stimulated macrophages). GM-CSF is secreted as a single chain glycoprotein containing 128 amino acids for human with a conserved disulfide bond. Human and murine GM-CSF share approx. 54% sequence homology and do not cross-react in bioactivity.