Merck Epidermal Growth Factor, human (hEGF)

Epidermal Growth Factor, human (hEGF)

recombinant (E. coli)

EGF, human

Quality Level

100

생물학적 소스

human

재조합

expressed in E. coli

assay

>95% (HPLC)

form

lyophilized

효능

<0.5 ng/mL EC50

분자량

6200 Da

포장

pkg of 100 μg

manufacturer/tradename

Roche

저장 조건

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

색상

white

UniProt 수납 번호

Q6QBS2

배송 상태

dry ice

저장 온도

−20°C

Gene Information

human ... EGF(1950)

Recombinant Epidermal Growth Factor, human (hEGF), is produced in E. coli and purified by standard chromatographic techniques. It is a 100μg white lyophilizate, filtered through 0.2μm pore size membrane, recombinant from E. coli.
Epidermal growth factor (EGF) is a small mitogenic polypeptide which is present in many mammalian species and is distributed throughout a wide number of tissues and body fluids EGF is produced in the tubular cells of the submaxillary gland of the mouse, in the acinar cells of the human submaxillary gland, and in the human duodenal glands.
Human EGF is identical to β-urogastrone, a polypeptide which was recognized and isolated on the basis of its ability to inhibit gastric acid secretion. 37 amino acids of the 53 amino acids comprising the longer urogastrone (human EGF) and mouse EGF are common to both peptides (70% homology) and the three disulfide bonds are formed in the same relative positions. The biological activity of human EGF is similar to that of mouse EGF. The cellular receptor for EGF is the best understood growth factor receptor. The oncogene v-erbB codes for a product homologous to a portion of the EGF receptor in which the EGF-binding domain has been deleted. Evidence exists suggesting that this truncation of the EGF receptor may lead to constitutive activation without requirement for ligand binding.
EGF stimulates the proliferation and differentiation of awide variety of cells of ectodermal and mesodermal origin (e.g., fibroblasts, glial cells, keratinocytes, epithelial cells, endothelial cells, chondrocytes). EGF is a constituent of many serum-free media formulations for a variety of cells.