Merck Nidogen-1 (NID1) human

Nidogen-1 (NID1) human

recombinant, expressed in HEK 293 cells, suitable for cell culture

NID, NID1, Entactin, NID-1

재조합

expressed in HEK 293 cells

assay

95% (SDS-PAGE)

form

lyophilized powder

technique(s)

cell culture | mammalian: suitable

저장 온도

−20°C

Nidogen-1 belongs to the nidogens family of glycoproteins. This family consists of nidogen-1 and nidogen-2, both of which are sulfated monomeric glycoproteins. Nidogen-1 is ubiquitously present in the synaptic and extra synaptic basal lamina. It is mapped to human chromosome 1q42.3. Nidogen-1 encodes a 139 kDa, three globular domains namely G1, G2 and G3 and has multiple epidermal growth factor repeats. Recombinant human nidogen-1 is expressed in human HEK 293 cells as a glycoprotein with a calculated molecular mass of 133.5 kDa (amino acids 29-1247). This protein is manufactured in human cells, with no serum. The human cells expression system allows human-like glycosylation and folding, and often supports higher specific activity of the protein. The protein is produced with no artificial tags.