Merck Fibronectin human

Fibronectin human

recombinant, expressed in HEK 293 cells, lyophilized powder, suitable for cell culture

FN, Cold-insoluble globulin, Fibronectin, CIG

Quality Level

200

생물학적 소스

human

재조합

expressed in HEK 293 cells

무균

sterile

assay

≥95% (SDS-PAGE)

form

lyophilized powder

분자량

259.5 kDa (The protein migrates as a higher band on SDS-PAGE due to glycosylation.)

포장

pkg of 200 μg

technique(s)

cell culture | mammalian: suitable

불순물

≤1 EU/μg endotoxin, tested

UniProt 수납 번호

P02751

배송 상태

ambient

저장 온도

−20°C

Gene Information

human ... FN1(2335)

Fibronectin (FN) is a glycoprotein with 5-9% carbohydrates present as N- and O-linked oligosaccharides. It belongs to the ligand glycoprotein family. The FN gene is localized to human chromosome 2q35. FN exists as two major isoforms, plasma-based soluble and extracellular matrix (ECM)-based insoluble form. FN is a multidomain protein and comprises repeats of FNI, FNII and FNIII repeats. It also has fibrin-binding sites and exists as a dimer. Alternative splicing of the FN1 gene leads to the generation of many isoforms. Recombinant human fibronectin is expressed in human 293 cells as a glycoprotein with a calculated molecular mass of 259.5 kDa. This protein is manufactured in human cells using an all-human production system with no serum. The human cells expression system allows human-like glycosylation and folding, and often supports better stability of the protein in culture.