Merck Human Laminin (pepsinized) Purified Protein

Human Laminin (pepsinized) Purified Protein

Quality Level

100

생물학적 소스

human

assay

≥95% (SDS-PAGE)

form

liquid

manufacturer/tradename

Chemicon®

농도

0.25 mg/mL

technique(s)

cell culture | mammalian: suitable

input

sample type mesenchymal stem cell(s)
sample type induced pluripotent stem cell(s)
sample type hematopoietic stem cell(s)
sample type: human embryonic stem cell(s)
sample type epithelial cells
sample type pancreatic stem cell(s)
sample type neural stem cell(s)

NCBI 수납 번호

NM_002291.1

UniProt 수납 번호

P07942

배송 상태

dry ice

저장 온도

−20°C
−70°C

Gene Information

human ... LAMB1(3912)

AG56P contains a mixture of human laminins containing the beta1 chain, principally laminin 1, 2, 3, 6, 8 & 10. This preparation is immunologically and biologically identical to intact human laminin. AG56P is suitable for use in ELISA, production of antiserum, cell adhesion and attachment assays, and neurite-stimulation assays. This pepsinized laminin preparation runs as twin bands migrating at approximately 160kDa and 130kDa by SDS-PAGE under reducing conditions.

Preparation: Chemicon′s purified human laminin is prepared from freshly frozen human placenta tissue that is homogenized in PBS with a blender and the mixture centrifuged. The pellet is then washed with 0.5M acetic acid, collected and subjected to and extended Pepsin A digestion. The pepsin digest is neutralized, centrifuged to clear debris, and applied to an antibody column containing mouse anti-human laminin monoclonal 4E10 monoclonal antibody {MAB1921}. The solubilized laminin solution is passed over the column, and utlimately the laminin is released via acidification with KSCN. Then the material is dialyzed against PBS. The antibody 4E10 is specific for laminin beta1 chain. This chain is found in Laminin′s 1,2,3,6,8,10 under the native conditions used to purify the material.