Merck SPITE Medium Supplement (100×)

SPITE Medium Supplement (100×)

liquid, sterile-filtered, BioReagent, suitable for cell culture

무균

sterile-filtered

Quality Level

400

제품 라인

BioReagent

form

liquid

저장 조건

protect from light

technique(s)

cell culture | mammalian: suitable

불순물

endotoxin, tested

배송 상태

ambient

저장 온도

2-8°C

Serum-free and reduced serum formulations often require purified forms of the factors normally supplied by serum that are suitable for in vitro growth and differentiation studies. These important factors include insulin, transferrin, selenium, pyruvate, and ethanolamine.

INSULIN is a polypeptide hormone that promotes the uptake of glucose and amino acids and may owe observed mitogenic effect to this property.

TRANSFERRIN is an iron-transport protein. Iron is an essential trace element, but can be toxic in the free form. To nourish cells in culture, it is supplied bound to transferrin in serum.

SELENIUM is an essential trace element normally provided by Serum.

SODIUM PYRUVATE has been shown to be beneficial as an additional energy source in some instances.

ETHANOLAMINE is a fatty acid that plays a significant role in the proliferation of hybridoma cells and frequently is added to supplements used for culturing these cells.

Nutritional studies indicate that the supplement components described are utilized by most mammalian cells. They enhance cell proliferation and decrease the serum requirement of many cell types. When the provided supplement is used with 2 to 4 percent serum, proliferation is reported to be similar to medium supplemented with 10 percent serum.