Merck Insulin-transferrin-sodium selenite media supplement

Insulin-transferrin-sodium selenite media supplement

γ-irradiated, lyophilized powder, BioXtra, suitable for cell culture

ITS Supplement

Quality Level

200

생물학적 소스

human plasma

무균

γ-irradiated

제품 라인

BioXtra

form

lyophilized powder

technique(s)

cell culture | mammalian: suitable

UniProt 수납 번호

P01308

배송 상태

ambient

저장 온도

−20°C

Gene Information

human ... INS(3630)

Serum-free and reduced serum formulations often require purified forms of the factors normally supplied by serum that are suitable for in vitro growth and differentiation studies. These important factors include insulin, transferrin, selenium, pyruvate, and ethanolamine.

INSULIN is a polypeptide hormone that promotes the uptake of glucose and amino acids and may owe observed mitogenic effect to this property.

TRANSFERRIN is an iron-transport protein. Iron is an essential trace element, but can be toxic in the free form. To nourish cells in culture, it is supplied bound to transferrin in serum.

SELENIUM is an essential trace element normally provided by Serum.

Nutritional studies indicate that the supplement components described are utilized by most mammalian cells. They enhance cell proliferation and decrease the serum requirement of many cell types. When the provided supplement is used with 2 to 4 percent serum, proliferation is reported to be similar to medium supplemented with 10 percent serum.