Merck Albumin, Acetylated from bovine serum

Albumin, Acetylated from bovine serum

protease free, for molecular biology

Quality Level

200

생물학적 소스

bovine

Grade

for molecular biology

제품 라인

BioReagent

form

solution

정제법

heat shock fractionation

포함

does not contain stabilizer or preservative

기원

USA origin

농도

20 mg/mL in H2O

technique(s)

FISH: suitable

불순물

HIV I and HIVII, HCV and HBsAg, tested negative

UniProt 수납 번호

P02769

외래 활성

DNase free
DNase, RNase, NICKase and protease, none detected
NICKase free
Protease free
RNase free

저장 온도

−20°C

Gene Information

bovine ... ALB(280717)

Serum albumin is the most extensive plasma protein in the circulatory system. It is produced within the liver and exported as a non-glycosylated protein to the blood plasma. The molecular weight of native serum albumin is around 66 kDa. Native serum albumin is a negatively charged protein.

Acetylation of bovine serum albumin particularly modifies lysine (Lys) residues with elevated frequency, with lesser degrees of modification of serine (Ser) and threonine (Thr) residues. MALDI-TOF mass spectrometry analysis of acetylated BSA has indicated an increased MW of about 3000, compared to native BSA (Ostatná, V. et al., J. Electroanalytical Chem., 821, 97-103 (2018)).. This acetylation process also derivatizes similar amino acid residues in any trace contaminating nucleases in the BSA, to inactivate that nuclease activity. This makes acetylated BSA useful in molecular biology applications where trace nuclease activity must be minimized.