Merck Trypsin solution from porcine pancreas

Trypsin solution from porcine pancreas

1 ×, sterile-filtered, BioReagent, suitable for cell culture, 2.5 g porcine trypsin per liter in Hanks′ Balanced Salt Solution with phenol red

생물학적 소스

Porcine pancreas

Quality Level

400

무균

sterile-filtered

제품 라인

BioReagent

form

solution

분자량

23.4 kDa

농도

1 ×

technique(s)

cell culture | mammalian: suitable

불순물

Porcine parvovirus, none detected (9 CFR)

pH

7.0-7.6

배송 상태

dry ice

저장 온도

−20°C

Trypsin solution from porcine pancreas has been used for keratinocyte isolation and staining. It has also been used as a component of CMF-Steinberg′s solution to replace heart tissues after centrifugation in a study to culture embryonic cardiomyocytes from Mexican axolotl.

For trypsin digestion of peptides, use a ratio of about 1:100 to 1:20 for trypsin:peptide. The typical use for this product is in removing adherent cells from a culture surface. The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture. Trypsins have also been used for the re-suspension of cells during cell culture, in proteomics research for digestion of proteins and in various in-gel digestions. Additional applications include assessing crystallization by membrane-based techniques and in a study to determine that protein folding rates and yields can be limited by the presence of kinetic traps.