Merck Trypsin solution from porcine pancreas
돼지 췌장에서 유래한 트립신 용액으로 세포 배양에 적합한 멸균 여과 제품입니다. 단백질 소화, 세포 부착 해제, 단백질 재결정화 연구 등에 활용됩니다. BioReagent 등급으로 품질이 우수하며, −20°C에서 보관합니다.
- 카탈로그번호
- T4424-xxxxx (2개 옵션)
- 브랜드
- Merck Sigma
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Merck Sigma · Merck Trypsin solution from porcine pancreas
Trypsin solution from porcine pancreas
1×, sterile-filtered, BioReagent, suitable for cell culture
2.5 g porcine trypsin per liter in Hanks′ Balanced Salt Solution with phenol red
제품 정보
| 항목 | 내용 |
|---|---|
| 생물학적 소스 | Porcine pancreas |
| Quality Level | 400 |
| 무균 여부 | Sterile-filtered |
| 제품 라인 | BioReagent |
| 형태 (Form) | Solution |
| 분자량 | 23.4 kDa |
| 농도 | 1× |
| 적용 기술 (technique) | Cell culture (mammalian): suitable |
| 불순물 | Porcine parvovirus, none detected (9 CFR) |
| pH | 7.0–7.6 |
| 배송 상태 | Dry ice |
| 저장 온도 | −20 °C |
제품 설명
Trypsin solution from porcine pancreas has been used for keratinocyte isolation and staining. It has also been used as a component of CMF‑Steinberg′s solution to replace heart tissues after centrifugation in a study to culture embryonic cardiomyocytes from Mexican axolotl.
For trypsin digestion of peptides, use a ratio of about 1:100 to 1:20 for trypsin:peptide.
The typical use for this product is in removing adherent cells from a culture surface. The concentration of trypsin necessary to dislodge cells from their substrate depends primarily on the cell type and the age of the culture.
Trypsins have also been used for:
- Re-suspension of cells during cell culture
- Proteomics research for protein digestion
- Various in-gel digestions
- Assessing crystallization by membrane-based techniques
- Studies on protein folding rates and kinetic traps
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