Merck Trypsin solution from porcine pancreas

Trypsin solution from porcine pancreas

1×, sterile-filtered, BioReagent, suitable for cell culture
2.5 g porcine trypsin per liter in Hanks′ Balanced Salt Solution with phenol red

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Trypsin solution from porcine pancreas has been used for keratinocyte isolation and staining. It has also been used as a component of CMF‑Steinberg′s solution to replace heart tissues after centrifugation in a study to culture embryonic cardiomyocytes from Mexican axolotl.

For trypsin digestion of peptides, use a ratio of about 1:100 to 1:20 for trypsin:peptide.
The typical use for this product is in removing adherent cells from a culture surface. The concentration of trypsin necessary to dislodge cells from their substrate depends primarily on the cell type and the age of the culture.

Trypsins have also been used for:

• Re-suspension of cells during cell culture
• Proteomics research for protein digestion
• Various in-gel digestions
• Assessing crystallization by membrane-based techniques
• Studies on protein folding rates and kinetic traps