Merck Minimum Essential Medium

Minimum Essential Medium

with Earle′s Balanced Salts, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

MEM, Minimum Essential Medium Eagle

Quality Level

500

설명

for research or for further manufacturing use

무균

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable
cell culture | stem cell: suitable

성분

NaHCO3: 2200 mg/L
Earle’s salts (5% CO2): yes
phenol red: 10.62 mg/L
L-glutamine: no

저장 온도

2-8°C

Minimum Essential Medium (MEM) with Earle′s Balanced Salts was developed by Harry Eagle as a result of his studies to determine the essential nutritional requirements of mouse L cells and HeLa cells in culture. He demonstrated that these cells require 13 amino acids and seven vitamins to grow and reproduce in vitro. MEM is a modification of Eagle′s earlier medium Basal Medium Eagle (BME), containing higher concentrations of the essential nutrients. This medium has demonstrated the ability to support a variety of normal and transformed cells in culture and contains Earle′s Balanced Salts, which makes it suitable for use in atmospheres charged with CO2 gas.