Merck Trypsin (1X)

Trypsin (1X)

sterile; γ-irradiated, 0.25% porcine trypsin (1:250) in HBSS, w/0.1% EDTA-Na2, w/o Ca++ and Mg++

Trypsin-EDTA solution

Quality Level

500

설명

for research or for further manufacturing use

무균

sterile; γ-irradiated

form

liquid

technique(s)

cell culture | mammalian: suitable

성분

phenol red: 10.620 mg/L
NaHCO3: 350.000 mg/L

배송 상태

dry ice

저장 온도

−20°C

Trypsin-EDTA solutions are used to release adherent cells from culture surfaces. They are generally composed of variable concentrations of natural porcine pancreas-derived trypsin and EDTA. Trypsin 1X solutions can range from 0.025% to 0.5%. The reasons for the range of concentrations are differences in required trypsin activity or potency; differences in incubation times; and cell line susceptibility. The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture. Various formulations should be tested to determine the best product for a specific application.