Merck EX-CELL 420 Serum-Free Medium for Insect Cells

EX-CELL® 420 Serum-Free Medium for Insect Cells

with L-glutamine, sterile-filtered, suitable for insect cell culture

Quality Level

500

설명

for research or for further manufacturing use

무균

sterile-filtered

form

liquid

품질

Drug Master File available

technique(s)

cell culture | insect: suitable

성분

glucose: 6.0 g/L
NaHCO3: 0.35 g/L
HEPES: no
L-glutamine: 1.0 g/L

배송 상태

ambient

저장 온도

2-8°C

EX-CELL 420 is a complete medium developed and optimized for the serum-free growth of Sf9 and Sf21 insect cell lines. Cells can be subcultured directly into EX-CELL 420 from serum-free or seum-supplemented media without adaptation. Cultures in EX-CELL 420 routinely reach cell densities greater than 1 × 107 cells/mL with greater than 95% viability. Suspension cultures can be maintained, without refeeding, for more than 10 days. Sf9 and Sf21 cells have been carried for more than 20 passages in EX-CELL 420 with no loss of viability. Protein expression and virus production are improved over serum-containing media.