Merck M2 medium

M2 medium

With HEPES, without penicillin and streptomycin, liquid, sterile-filtered, suitable for mouse embryo cell culture

Quality Level

400

무균

sterile-filtered

form

liquid

저장 조건

protect from light

technique(s)

cell culture | embryo: suitable

불순물

endotoxin, tested

성분

phenol red: 0.0106 g/L
sodium pyruvate: 0.0363 g/L
HEPES: 5.42726 g/L
glucose: 1.0 g/L (Dextro)
NaHCO3: 0.35 g/L

배송 상태

ambient

저장 온도

2-8°C

Recommended for manipulation of mouse embryos at ambient temperature. M2 and M16 Medium are common media for in vitro culture of preimplantation stage embryos. It is a modified Krebs-Ringer bicarbonate solution, which is very similar to Whitten′s Medium. M16 contains pyruvate and lactate as energy sources since preimplantation embryos do not utilize glucose efficiently.

M2 Medium is a further modification of M16 that substitutes HEPES buffer in place of some of the bicarbonate. M2 is used for collecting and handling embryos for prolonged periods outside a CO2 incubator.