Merck Minimum Essential Medium Eagle

Minimum Essential Medium Eagle

With Earle′s salts, L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

MEM

Quality Level

400

무균

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

불순물

endotoxin, tested

성분

HEPES: no
NaHCO3: yes
L-glutamine: yes
Earle’s salts (5% CO2): yes
sodium pyruvate: no
phenol red: yes

배송 상태

ambient

저장 온도

2-8°C

Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media. Early attempts to cultivate normal mammalian fibroblasts and certain subtypes of HeLa cells revealed they had specific nutritional requirements that could not be met by Eagle′s Basal Medium (BME). Subsequent studies using these and other cells in culture indicated additions to BME could be made to aid growth of a wider variety of fastidious cells.

MEM, which incorporates these modifications, includes higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. MEM has been used for cultivation of a wide variety of cells grown in monolayers. Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks′ or Earle′s salts has broadened the usefulness of this medium.