Merck In Vitro Toxicology Assay Kit, MTT based

In Vitro Toxicology Assay Kit, MTT based

사용

kit sufficient for 1,000 tests

Quality Level

200

포장

pkg of 1 kit

저장 조건

dry at room temperature

λmax

570 nm

application(s)

cell analysis
detection

detection method

colorimetric

저장 온도

2-8°C

This kit is designed for determining cell number/ cell viability spectrophotometrically as a function of mitochondrial activity in living cells. The MTT method is simple, accurate and yields reproducible results. The key component is (3- [4,5- dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide) or MTT. Solutions of MTT, dissolved in medium or balanced salt solutions without phenol red, are yellowish in color. Mitochondrial dehydrogenases of viable cells cleave the tetrazolium ring, yielding purple formazan crystals which are insoluble in aqueous solutions. Absorbance of converted dye is measured at a wavelength of 570nm.