Merck Cell Proliferation Kit II (XTT)

Cell Proliferation Kit II (XTT)

liquid, pkg of 1 kit, suitable for cell analysis, suitable for tissue culture

xtt

Quality Level

100

form

liquid

사용

sufficient for ≤2,500 tests

포장

pkg of 1 kit

manufacturer/tradename

Roche

저장 조건

protect from light

technique(s)

tissue culture: suitable

λmax

450-500 nm

application(s)

cell analysis
detection

detection method

colorimetric

배송 상태

dry ice

저장 온도

−20°C

The Cell Proliferation Kit II (XTT) is a colorimetric assay for the nonradioactive quantification of cellular proliferation, viability, and cytotoxicity. Sample material is either adherent or suspension cells cultured in 96-well microplates.

Colorimetric assays analyze the number of viable cells by the cleavage of tetrazolium salts added to the culture medium. This technique requires neither washing nor harvesting of cells, and the complete assay, from microculture to data analysis by an ELISA reader, is performed in the same microplate.
More recently, the tetrazolium salt XTT was described. In contrast to MTT, the cleavage product of XTT is soluble in water; therefore, a solubilization step is not required. The tetrazolium salt XTT is cleaved to formazan by a complex cellular mechanism. This bioreduction occurs in viable cells only, and is related to NAD(P)H production through glycolysis. Therefore, the amount of formazan dye formed directly correlates to the number of metabolically active cells in the culture.