Merck PKH67 Green Fluorescent Cell Linker Midi Kit for General Cell Membrane Labeling

PKH67 Green Fluorescent Cell Linker Midi Kit for General Cell Membrane Labeling

Distributed for Phanos Technologies

포장

pkg of 1 kit

Quality Level

100

manufacturer/tradename

Distributed for Phanos Technologies

저장 조건

protect from light

technique(s)

flow cytometry: suitable

형광

λex 490 nm; λem 502 nm (PKH67 dye)

application(s)

cell analysis
detection

detection method

fluorometric

배송 상태

ambient

저장 온도

room temp

This kit is for general cell membrane labeling. PKH67 has a longer aliphatic carbon tail than PKH1 and PKH2, two other green dyes previously described for in vitro and in vivo cell tracking. Based on the longer tail length, in-house studies have consistently shown reduced cell-cell transfer for PKH67 as compared to PKH2.
Slow loss of fluorescence has been observed in in vivo studies using PKH1 and PKH2. PKH67 may exhibit similar properties since this behavior appears to be characteristic of green cell linker dyes, but not red cell linker dyes. Correlation of in vitro cell membrane retention with in vivo fluorescence half life in non-dividing cells predicts an in vivo fluorescence half life of 10-12 days for PKH67. Other green cell linker dyes with similar half lives have been used to monitor in vivo lymphocyte and macrophage trafficking over periods of 1-2 months, suggesting that PKH67 will also be useful for in vivo tracking studies of moderate length.