Merck Human iPSC Derived AFE Progenitors

Human iPSC Derived AFE Progenitors

제품 개요

Cryopreserved human iPSC derived anterior foregut endoderm (AFE) progenitor cells that express Sox2, Pax9, and EpCAM.

기술(Technique)

• Cell culture
• Stem cell: suitable

응용(Application)

• Cell analysis

제품 설명

The anterior foregut endoderm (AFE) gives rise to therapeutically relevant cell types in tissues such as the lung, esophagus, salivary glands, thymus, parathyroid, and thyroid.
These cryopreserved AFE progenitor cells were derived from integration-free human iPS cells (Cat. No. SCC271) reprogrammed using Simplicon RNA reprogramming technology (Cat. No. SCR550) under chemically defined, serum-free culture conditions.

The AFE cells are double positive for:

• Sox2 (A, C; Sigma AB5603A4; 1:50)
• Pax9 (B, C; Abcam ab28538; 1:200)
• Approximately 20–30% are double positive for EpCAM (D, F; Sigma MAB4444; 1:500) and Pax9 (E, F)

관련 시스템

Human iPSC derived AFE progenitors are a component of the 3dGRO™ Human Lung Organoid Culture System, which enables differentiation of human iPS cells into mature lung organoids that structurally resemble in vivo branching airway and early alveolar structures.

3dGRO™ Human Lung Organoid Culture System 구성품 (별도 판매)

1. Definitive Endoderm Induction Medium (SCM302)
2. Anterior Foregut Endoderm Induction Medium I (SCM305)
3. Anterior Foregut Endoderm Induction Medium II (SCM306)
4. 3dGRO™ Lung Organoid Branching Medium (SCM307)
5. 3dGRO™ Lung Organoid Maturation Medium (SCM308)
6. Human iPSC Derived AFE Progenitors (SCC301)

연구 카테고리

• Cell Culture
• Stem Cell Research

제품 사양