Thermo Fisher Scientific Histone H1 (Pan Nuclear Marker) Monoclonal Antibody (AE-4)
Histone H1 단백질을 인식하는 AE-4 클론 단일클론 항체로, 핵 마커로 활용 가능. Western blot, IHC, ICC/IF, Flow cytometry 등 다양한 응용에 적합. 인간, 마우스, 랫트 시료에 반응하며, 단백질 A/G 정제 및 PBS 용액 형태로 제공.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | AE-4 |
| Immunogen | Nuclei of human leukemia biopsy cells (Histone H1 family: UniProt IDs P16401-1, P16403-1, Q02539-1, P07305-1; antigen range 1–226 aa depending on isoform) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
This locus is the ortholog of a mouse protein-coding gene that displays characteristics of a linker histone and is expressed in nuclei of late maturing spermatids. The human locus is expressed; however, the open reading frame has been disrupted by a frameshift, so it is unlikely to encode a functional protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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