Thermo Fisher Scientific WTAP Monoclonal Antibody (OTI6C3), TrueMAB
WTAP 단백질을 인식하는 Mouse IgG1 단일클론 항체로, Western blot 및 IHC(P) 실험에 적합합니다. 인간 유래 재조합 단백질(aa 2-106)을 면역원으로 제작되었으며, 고순도 친화 크로마토그래피 정제 제품입니다. 연구용으로만 사용 가능합니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:2,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI6C3 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 2–106 of human WTAP produced in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 1% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | −20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Wilms' tumor (WT) is an embryonal malignancy of the kidney that affects approximately 1 in 10,000 infants and occurs in both sporadic and inherited forms. The Wilms' tumor protein (WT1) binds the DNA sequence GCGGGGGCG, a recognition element common to the Egr family of Zn²⁺ finger transcriptional activators, functioning as a transcriptional repressor.
WTAP (Wilms tumor 1-associating protein) is a ubiquitously expressed nuclear protein that interacts with WT1 and may be involved in regulating mRNA splicing. WTAP localizes to nuclear speckles, where it regulates the G2/M cell cycle transition by binding to the 3′ UTR of cyclin A2, enhancing its stability. Additionally, WTAP inhibits expression of WT1 target genes and impairs the ability of WT1 to bind DNA. Two isoforms of WTAP exist due to alternative splicing events.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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