Thermo Fisher Scientific Cortactin Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:2,000–1:10,000

Immunoprecipitation (IP)

• Tested Dilution: 2–5 µg/mg lysate

Product Specifications

Product Specific Information

• Recommended shelf life: 1 year from date of receipt
• Based on 100% sequence identity, predicted to react with Chicken

Target Information

Cortactin (Cttn) is a ubiquitous actin-binding protein originally identified as a substrate for Src. It contributes to actin cytoskeleton organization and cell shape, playing a role in lamellipodia formation and cell migration. Cortactin accumulates in actin-enriched peripheral structures, facilitating actin network formation.

It contains four major domains:

• N-terminal acidic (NTA) domain
• Tandem repeats domain
• C-terminal proline-rich domain
• SH3 domain

NTA associates with the Arp2/3 and WASP complex at F-actin branches. Cortactin promotes cell motility and invasion, including a critical role in invadopodia, actin-rich protrusions involved in ECM degradation by cancer cells. It is phosphorylated by Src family kinases at Y421, Y466, and Y482, and by Erk family kinases at S405 and S418.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.