Thermo Fisher Scientific Expi293 Inducible GnTI- Expression System Kit

Gibco™ Expi293™ Inducible GnTI- Expression System Kit

The Expi293 Inducible GnTI- Expression System Kit is part of the Expi293 platform for rapid, high-yield transient protein expression from mammalian 293 cells.
This system utilizes engineered Expi293F cells lacking N-acetylglucosaminyltransferase I (GnTI) activity, resulting in the absence of complex N-glycans. These cells stably express the tetracycline repressor (TetR) protein, enabling tetracycline-regulated protein expression.

The Expi293F Inducible GnTI- cell line was established through selective knockout of the GnTI gene and stable integration of the regulatory plasmid pcDNA6/TR, encoding the TetR gene under the control of the human CMV promoter.
This cell line is a powerful tool for tetracycline-inducible, high-yield expression of homogeneously glycosylated recombinant proteins.

주요 특징

• 균일한 N-당화 단백질 발현
• 테트라사이클린 유도형 단백질 발현, 매우 낮은 기저 발현 수준
• Expi293F 모세포주와 동등한 단백질 수율 (최대 1 g/L)
• pcDNA5/TO 발현 벡터 포함 (유전자 발현 조절용)
• Expi293 Expression Medium을 이용한 고밀도 부유 배양
• ExpiFectamine 293 reagent 및 특수 트랜스펙션 강화제 사용으로 고효율 형질감염

구성품

• 동결된 Expi293F Inducible GnTI- 세포 1 바이알
• Expi293 Expression Medium 1 L
• ExpiFectamine 293 Transfection Kit (1 L 배양분량)
• Opti-Plex Complexation Buffer 100 mL
• PNGase F Glycan Cleavage Kit 1개
• pcDNA5/TO Mammalian Expression Vector Kit 1개
• Tetracycline Hydrochloride 500 mg
• pRABBIT IgG IRES-EmGFP Positive Control Vector 1개

모든 구성품은 개별 구매도 가능합니다.

사양

제품 이미지

(이미지 없음)