Thermo Fisher Scientific NSUN2 Polyclonal Antibody
Human NSUN2 단백질을 인식하는 Rabbit Polyclonal Antibody로, WB 및 IHC(P) 검증 완료. 항원 친화 크로마토그래피로 정제되었으며, 0.3 mg/mL 농도의 액상 형태. PBS와 글리세롤 완충액에 보관되며 연구용으로 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human NSUN2. Recombinant protein control fragment (Product # RP-96791) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.3 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Wet ice |
| RRID | AB_2644820 |
Product Specific Information
Immunogen sequence:
PFVFIPEDDP LFPPIEKFYA LDPSFPRMNL LTRTTEGKKR QLYMVSKELR NVLLNNSEKM KVINTGIKVW CRNNSGEEFD CAFRLAQ
Highest antigen sequence identity to the following orthologs:
- Mouse: 100%
- Rat: 100%
Target Information
NSUN2 encodes a methyltransferase that catalyzes the intron-dependent formation of 5-methylcytosine at C34 of tRNA-leu(CAA).
Maturation of cytoplasmic tRNAs includes splicing of introns located 1 nucleotide 3′ from the anticodon in all intron-containing tRNA genes.
In tRNA-leu(CAA), the first position of the anticodon, C34, is converted to 5-methylcytosine — a modification essential for stabilizing anticodon-codon pairing and accurate mRNA translation.
Mutations in NSUN2 are associated with autosomal recessive mental retardation (MRT5).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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