Thermo Fisher Scientific AMBRA1 Monoclonal Antibody (6B11D1)

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:2,000

Flow Cytometry (Flow)

• Tested Dilution: 1:200–1:400

Product Specifications

Product Specific Information

This antibody has been tested in indirect ELISA.

Target Information

Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway, is important for normal growth control and may be defective in tumor cells. It plays a role in maintaining cellular nutrients under starvation and in normal turnover of cytosolic components.
Beclin-1, a principal regulator of autophagosome formation, is regulated by Ambra1. Ambra1 associates with Beclin-1 through a region near its center as determined by yeast two-hybrid assay.
Null mutations in this gene in mice result in embryonic lethality with severe neural tube defects, autophagy impairment, accumulation of ubiquitinated proteins, unbalanced cell proliferation, and excessive apoptotic death.
Down-regulation of Ambra1 in cultured cells through RNA interference decreases rapamycin- and nutrient starvation-induced autophagy. Multiple isoforms of Ambra1 are known to exist.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.